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ABSENCE OF SEASONAL EFFECT ON THE IMMUNOMODULATORY ACTION OF BRAZILIAN PROPOLIS ON NATURAL KILLER ACTIVITY J. Venom. Anim. Toxins
SFORCIN,J. M.; KANENO,R.; FUNARI,S. R. C..
Propolis, a beehive product widely used in folk medicine as an antiinflammatory agent, has been attracting researchers' attention to scientifically elucidate its biological properties and therapeutic activities. The aim of this paper was to study the possible effect of propolis on natural killer activity, since propolis immunomodulatory action has been suggested, especially on non-specific immunity. Propolis was produced by africanized honeybees (Apis mellifera L.), collected throughout a whole year, and pooled by season. Hydroalcoholic solutions of propolis were prepared with each pool and administered to rats by gavage over three days. Natural killer activity of non-adherent spleen cells was evaluated by the 51Cr-release cytotoxicity assay against Yac-1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propolis; Season; Immunomodulation; Natural killer activity; Cytotoxic assay.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100003
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Activity of Brazilian propolis against Aeromonas hydrophila and its effect on Nile tilapia growth, hematological and non-specific immune response under bacterial infection Anais da ABC (AABC)
ORSI,RICARDO O.; SANTOS,VIVIAN G. DOS; PEZZATO,LUIZ E.; CARVALHO,PEDRO L.P.F. DE; TEIXEIRA,CAROLINE P.; FREITAS,JAKELINE M.A.; PADOVANI,CARLOS R.; SARTORI,MARIA M.P.; BARROS,MARGARIDA M..
ABSTRACT The effect of the ethanolic extract of propolis (EEP) on Aeromonas hydrophila was analyzed by determination of minimum inhibitory concentration (MIC). Then, the effects of crude propolis powder (CPP) on growth, hemato-immune parameters of the Nile tilapia, as well as its effects on resistance to A. hydrophila challenge were investigated. The CPP (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0%) was added to the diet of 280 Nile tilapia (50.0 ± 5.7 g fish-1). Hemato-immune parameters were analyzed before and after the bacterial challenge. Red blood cell, hematocrit, hemoglobin, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and hydrogen peroxide (H2O2) and nitric oxide (NO) were evaluated. The MIC of the EEP was 13% (v/v) with a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Health; Hematology; Immunology; Oreochromis niloticus; Propolis; Stress.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401785
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Analysis of the composition of Brazilian propolis extracts by chromatography and evaluation of their in vitro activity against gram-positive bacteria BJM
Sawaya,Alexandra C. H. F.; Souza,Kirllian S.; Marcucci,Maria C.; Cunha,Ildenize B.S.; Shimizu,Mario T..
Brazilian propolis from São Paulo state was submitted to extraction using several solvents, resulting in extracts with different composition. These extracts were submitted to Thin Layer Chromatography (TLC). Bioauthographic analysis of the TLC plates identified fractions with inhibitory activity, which were then analysed by High Performance Liquid Chromatography (HPLC). In vitro assays, commonly used to evaluate the activity of propolis against Gram-positive bacteria, were compared to determine which rendered the most consistent results. The bactericidal activity of these extracts were analysed by Serial Dilution in Tubes and Agar Plate Diffusion. Serial Dilution in Tubes obtained the most consistent results, with the Minimal Bactericidal Concentration of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propolis; Thin layer chromatography; Bioauthography; High performance liquid chromatography; Extraction methods; Minimal bactericidal concentration.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100017
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Antibacterial activity of Apis mellifera L. propolis collected in three regions of Kenya J. Venom. Anim. Toxins incl. Trop. Dis.
Muli,E. M.; Maingi,J. M..
The present study aimed at investigating the susceptibility of the microorganisms Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis to ethanolic extracts of propolis (EEP) from three regions of Kenya (Taita, Tana and Samburu). Propolis was extracted using four different concentrations of ethanol: pure, 70%, 50%, and 30%. Ethanol (70%) and Streptomycin were used as controls. The agar diffusion method using filter paper disks was employed. Antibacterial activity was determined as an equivalent of the inhibition zones diameters (in millimeters) after incubation at 37°C for 24h. Significant differences in the antibacterial activities of propolis were observed among the three regions, depending on the test...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apis mellifera; Propolis; Antibacterial agents; Disk diffusion antimicrobial tests.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300008
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Antibacterial activity of propolis collected in different regions of Brazil J. Venom. Anim. Toxins incl. Trop. Dis.
Gonsales,G. Z.; Orsi,R. O.; Fernandes Júnior,A.; Rodrigues,P.; Funari,S. R. C..
The aim of this study was to investigate the antibacterial activity of propolis samples from Goiás, Paraná and São Paulo States, Brazil, and their flavonoids content. Ethanolic extracts of propolis (EEP) were prepared (30g of propolis in 70% ethanol), and the microorganisms Staphylococcus aureus and Escherichia coli were tested. The methodology employed was agar diffusion using filter paper discs. Ampicillin and tetracycline were used as controls. Antibacterial activity was determined by the reading of inhibition zone diameters (mm) after 24 hours incubation at 37°C. Results demonstrated that EEP inhibited the growth of Staphylococcus aureus but not that of Escherichia coli. Tetracycline and ampicillin showed an efficient action against both bacteria....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propolis; Antibacterial activity; Flavonoids.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200009
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Anti-inflammatory activity of propolis extracts: a review J. Venom. Anim. Toxins
Almeida,E. C. de; Menezes,H..
Since primeval times, the inflammatory process has been described in many different ways. Several anti-inflammatory therapies have been used in different biological models. However, in a recent "back to nature move", modern man is searching for natural products with medicinal properties, particularly those obtained from plants and bees. Propolis has been used in folk medicine for a very long time. The many compounds present in propolis require investigation.Physical-chemical analysis studies have not sufficiently established quality standards of propolis containing products. These standards should depend especially on their different pharmacological activities. There are few studies reporting on the in vitro anti-inflammatory activity of propolis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propolis; Anti-inflammatory activity; Bees; Apis mellifera; Natural products.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200002
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Antioxidant activity of bee products added to water in tebuconazole-exposed fish Neotropical Ichthyology
Ferreira,Daiane; Unfer,Taís Cristina; Rocha,Hélio Carlos; Kreutz,Luiz Carlos; Koakoski,Gessi; Barcellos,Leonardo José Gil.
An experiment was conducted to evaluate the potential of honey, propolis, and bee pollen for the reversal of lipid peroxidation induced by tebuconazole (TEB) in South American catfish (Rhamdia quelen), in which the concentration of thiobarbituric acid reactive substances (TBARS), the activity of the antioxidant enzyme glutathione-S-transferase (GST) and the concentrations of non-enzymatic antioxidants, reduced glutathione (GSH), ascorbic acid, and non-protein thiols were assessed. Honey (0.125 g L-1) and bee pollen (0.05 g L-1) added to the water reverse the production of TBARS induced by TEB, while propolis demonstrated a pro-oxidant effect, inducing an increase in TBARS production. The data presented herein suggest that the addition of water to honey and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Bee pollen; Honey; Lipid peroxidation; Propolis; Tebuconazole.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000100021
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Antiproliferative effects of Tubi-bee propolis in glioblastoma cell lines Genet. Mol. Biol.
Borges,Kleiton Silva; Brassesco,María Sol; Scrideli,Carlos Alberto; Soares,Ademilson Espencer Egea; Tone,Luiz Gonzaga.
Propolis is a resin formed by a complex chemical composition of substances that bees collect from plants. Since ancient times, propolis has been used in folk medicine, due to its biological properties, that include antimicrobial, anti-inflammatory, antitumoral and immunomodulatory activities. Glioblastoma is the most common human brain tumor. Despite the improvements in GBM standard treatment, patients' prognosis is still very poor. The aim of this work was to evaluate in vitro the Tubi-bee propolis effects on human glioblastoma (U251 and U343) and fibroblast (MRC-5) cell lines. Proliferation, clonogenic capacity and apoptosis were analyzed after treatment with 1 mg/mL and 2 mg/mL propolis concentrations for different time periods. Additionally,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glioblastoma; Propolis; Temozolomide; U251; U343.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200024
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Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress BJM
Sá,Rafael A. de; Castro,Frederico A.V. de; Eleutherio,Elis C.A.; Souza,Raquel M. de; Silva,Joaquim F.M. da; Pereira,Marcos D..
Propolis is a natural product widely used for humans. Due to its complex composition, a number of applications (antimicrobial, antiinflammatory, anesthetic, cytostatic and antioxidant) have been attributed to this substance. Using Saccharomyces cerevisiae as a eukaryotic model we investigated the mechanisms underlying the antioxidant effect of propolis from Guarapari against oxidative stress. Submitting a wild type (BY4741) and antioxidant deficient strains (ctt1∆, sod1∆, gsh1∆, gtt1∆ and gtt2∆) either to 15 mM menadione or to 2 mM hydrogen peroxide during 60 min, we observed that all strains, except the mutant sod1∆, acquired tolerance when previously treated with 25 µg/mL of alcoholic propolis extract. Such a treatment reduced the levels of ROS...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propolis; Antioxidant; Oxidative stress; Saccharomyces cerevisiae.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300051
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Candida sp in the oral cavity with and without lesions: maximal inhibitory dilution of Propolis and Periogard Rev. Microbiol.
Azevedo,Rosa Vitória Palamin; Komesu,Marilena Chinalli; Candido,Regina Celia; Salvetti,Cristiane; Rezende,Fausto Hanaoka Caetano.
Fifty individuals of both sexes aged on average 45.2 years were evaluated at the Semiology Clinic of FORP-USP in order to isolate and identify yeasts from the oral cavity, with and without lesions, and to determine the maximal inhibitory dilution (MID) of the commercial products Propolis (Apis-Flora) and Periogard (Colgate) against the strains isolated. Yeasts of the genus Candida were detected in the saliva of 9/19 (47.4%) individuals with a clinically healthy mouth, 18/22 (81.8%) of individuals with oral lesions, and in 4/9 (44.4%) of patients with deviation from normality, and were detected in 19/22 (86.4%) of the lesions. In the group with oral candidiasis, we isolated in tongue and lesion, respectively for each specie: C.tropicalis (8% and 10.7%),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oral candidiasis; Propolis; Chlorhexidine; Yeasts.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400008
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Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus BJM
Barrientos,Leticia; Herrera,Christian L.; Montenegro,Gloria; Ortega,Ximena; Veloz,Jorge; Alvear,Marysol; Cuevas,Alejandro; Saavedra,Nicolás; Salazar,Luis A..
Propolis is a non-toxic natural substance with multiple pharmacological properties including anticancer, antioxidant, fungicidal, antibacterial, antiviral, and anti-inflammatory among others. The aim of this study was to determine the chemical and botanical characterization of Chilean propolis samples and to evaluate their biological activity against the cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. Twenty propolis samples were obtained from beekeeping producers from the central and southern regions of Chile. The botanical profile was determined by palynological analysis. Total phenolic contents were determined using colorimetric assays. Reverse phase HPLC and HPLC-MS were used to determine the chemical composition. The minimum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propolis; Botanical characterization; Chemical characterization; Streptococcus mutans; Streptococcus sobrinus.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200038
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Chemical characterization, antioxidant and antimicrobial activity of propolis obtained from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula stingless bees BJMBR
Torres,A.R.; Sandjo,L.P.; Friedemann,M.T.; Tomazzoli,M.M.; Maraschin,M.; Mello,C.F.; Santos,A.R.S..
In this study, we investigated the chemical composition, and antioxidant and antibacterial properties of ethanolic extracts of propolis (EEP) from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula. Chemical composition of EEP was determined by colorimetry and chromatographic (HPLC-DAD and UPLC-Q/TOF-MS/MS) analysis. Antimicrobial activity of EEP was evaluated against gram-positive (S. aureus, methicillin-resistant S. aureus, E. faecalis) and gram-negative (E. coli and K. pneumoniae) bacteria by the minimal inhibitory concentration (MIC) test using the microdilution method. Furthermore, the growth curve and integrity of cell membrane of S. aureus and E. coli were investigated using standard microbiological methods. HPLC-DAD analysis showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bees; Propolis; Antimicrobial activity; S. aureus; M. quadrifasciata quadrifasciata; T. angustula.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600617
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Chemopreventive Effect of Brazilian Green Propolis on Experimental Dermal Carcinogenesis in Murine Model International Journal of Morphology
Pereira-Filho,Rose Nely; Batista,Fellipe Santos; Ribeiro,Danielle Rodrigues; Melo,Genecy Calado de; Reis,Francisco Prado; Melo,Allan Ulisses Carvalho de; Gomes,Margarete Zanardo; Cardoso,Juliana Cordeiro; Albuquerque Júnior,Ricardo Luiz Cavalcanti de.
The aim of this study was to assess the effect of oral administration of Hydroalcoholic Extract of Green Propolis (HEGP) on dermal carcinogenesis in rodent model. For the biological assay, we used 36 mice, assigned into 6 groups (n=6): CTR (treated with 100 mg/kg HEGP and no tumor induction), TUM (treated with water and tumor induction), GP10 (treated with 10 mg/kg HEGP and tumor induction), GP50 (treated with 50 mg/kg HEGP and tumor induction) and GP100 (treated with 100 mg/kg HEGP and tumor induction). Cancer induction was performed in the back of the mice by topical application of DMBA. After 16 weeks, mice were euthanized and their backs were submitted to post-mortem histological analysis. The mean number of lesions developed in TUM (4.14±0.89) was...
Tipo: Journal article Palavras-chave: Propolis; Skin cancer.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200024
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Effect of Bovine Type-I Collagen-Based Films Containing Red Propolis on Dermal Wound Healing in Rodent Model International Journal of Morphology
Albuquerque-Júnior,Ricardo Luiz Cavalcanti de; Barreto,André Luiz Santos; Pires,Jônatas Almeida; Reis,Francisco Prado; Lima,Sônia Oliveira; Ribeiro,Maria Amália Gonzaga; Cardoso,Juliana Cordeiro.
Propolis, an apitherapic product widely employed in natural medicine, presents various biological properties, such as bactericidal and anti-inflammatory activities, but only few reports have looked at the Brazilian red propolis variety. Collagen films have been employed to improve wound healing and some studies have investigated the effect of natural products incorporated into these films. Thus, the aim of this study was to analyze the effect of collagen-based films containing red propolis on wound healing. Therefore, wounds performed in the back of rats were dressed with collagen-based films (G2), and collagen-based films containing red propolis (G3); undressed worked as control (G1). After 3, 7 and 14 days the animals were euthanized, and the wounds were...
Tipo: Journal article Palavras-chave: Propolis; Wound healing; Occlusive dressings; Type-I collagen.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400025
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Effect of Chilean propolis on cariogenic bacteria Lactobacillus fermentum Ciencia e Investigación Agraria
Saavedra,Nicolás; Barrientos,Leticia; Herrera,Christian L; Alvear,Marysol; Montenegro,Gloria; Salazar,Luis A.
Dental caries is an infectious disease of worldwide public health concern. Among the bacteria involved in this pathology are Streptococcus mutans, Streptococcus sobrinus and organisms belonging to the genera Actinomyces and Lactobacillus. The pharmaceutical industry is focussing on the discovery of new antibacterial products after a greater resistance to those already known. Propolis has been used since ancient times, so their effects against various microorganisms have been already investigated. In our study, we evaluated the antimicrobial effect of 6 commercial ethanolic propolis extracts on the bacterium Lactobacillus fermentum. This bacterium was isolated after its identification by Polymerase Chain Reaction using species specific primers, and after...
Tipo: Journal article Palavras-chave: Lactobacillus fermentum; Dental caries; Propolis; Antibacterial activity.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000100011
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Effect of propolis extract on guines pig lung mast cell J. Venom. Anim. Toxins incl. Trop. Dis.
Orsi,R. O.; Sforcin,J. M.; Funari,S. R. C.; Gomes,J. C..
The direct effect of ethanolic extract of propolis on guinea pig lung cell suspension containing mast cells, as well as its influence on the histamine release induced by antigen (ovoalbumin 10 mug/ml) and ionophore A 23187 (3 muM) were investigated. Propolis ethanolic extract (300 mug/ml) increased the histamine release in guinea pig lung suspension containing mast cells by a cytotoxic effect. Lower concentrations of propolis had no effect on histamine release. Our results demonstrated that propolis (3, 10, 30, and 100 mug/ml) shows no significant effect on the histamine release induced by ionophore and antigen. Based on these results, we suggest that propolis could directly activate mast cells, promoting inflammatory mediators release by cytotoxic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mast cells; Allergy; Histamine; Propolis.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100009
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Effects of propolis from Brazil and Bulgaria on Salmonella serovars J. Venom. Anim. Toxins incl. Trop. Dis.
Orsi,R. O.; Sforcin,J. M.; Funari,S. R. C.; Fernandes-JR.,A.; Rodrigues,P.; Bankova,V..
Propolis shows biological properties such as antibacterial action. This bee product has a complex chemical composition, which depends on the local flora where it is produced. Salmonella serovars are responsible for human diseases that range from localized gastroenteritis to systemic infections. The aim of the present study was to investigate the susceptibility of Salmonella strains, isolated from food and infectious processes, to the antibacterial action of Brazilian and Bulgarian propolis, as well as to determine the behavior of these bacteria, according to the incubation period, in medium plus propolis. Dilution of ethanolic extract of propolis in agar was the used method. Brazilian and Bulgarian propolis showed an antibacterial action against all...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propolis; Susceptibility profile; Salmonella; Food; Infection.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400006
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Efficacy of Argentine propolis formulation for topical treatment of canine otitis externa Arq. Bras. Med. Vet. Zootec.
Lozina,L.A; Peichoto,M.E; Boehringer,S.I; Koscinczuk,P; Granero,G.E; Acosta,O.C.
The therapeutic effects of Argentine propolis ear drop formulation on canine otitis externa were evaluated. Forty-eight dogs with symptoms of otitis externa were randomly assigned to double-blinded, controlled clinical trial to evaluate the efficacy of topical formulation with propolis versus a topical placebo in the treatment of otitis externa. The propolis preparation and placebo were administrated into both external ear canals, twice daily for 14 days. Throughout the study, clinical examination and microbiological analysis of dogs ear exudates were made. The most frequent microorganisms isolated in culture media were: Malassezia pachydermatis (54.2%), Staphylococcus aureus (43.8%), coagulase-negative Staphylococcus (25.0%), Pseudomonas aeruginosa...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Propolis; Glicerin-propylenglycol; Otitis externa.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600010
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Evaluation of the in vitro antimicrobial activity of an ethanol extract of Brazilian classified propolis on strains of Staphylococcus aureus BJM
Pamplona-Zomenhan,Lucila Coelho; Pamplona,Beatriz Coelho; Silva,Cely Barreto da; Marcucci,Maria Cristina; Mimica,Lycia Mara Jenné.
Staphylococcus aureus (S. aureus) is one of the most frequent causes of hospital acquired infections. With the increase in multiple drug resistant strains, natural products such as propolis are a stratagem for new product discovery. The aims of this study were: to determine the in vitro antimicrobial activity of an ethanol extract of propolis; to define the MIC50 and MIC90 (Minimal Inhibitory Concentration - MIC) against 210 strains of S. aureus; to characterize a crude sample of propolis and the respective ethanol extract as to the presence of predetermined chemical markers. The agar dilution method was used to define the MIC and the high performance liquid chromatography (HPLC) method was used to characterize the samples of propolis. MIC results ranged...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propolis; Staphylococcus aureus; Antimicrobial Activity.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400002
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Immunomodulatory action of propolis on macrophage activation J. Venom. Anim. Toxins
ORSI,R.O.; FUNARI,S.R.C.; SOARES,A.M.V.C.; CALVI,S.A.; OLIVEIRA,S.L.; SFORCIN,J.M.; BANKOVA,V..
Propolis has been the subject of several recent studies, with the aim of elucidating its biological and pharmacological properties. Propolis has a well-known antimicrobial activity as well as antioxidant, antitumoral, antiinflammatory, and regenerative properties, but literature about its effects on the immune response is scarce. The goal of this work was to evaluate the propolis effect on macrophage activation by oxygen (H2O2) and nitrogen (NO) metabolite determination. Propolis was produced by africanized honeybees and hydroalcoholic solutions were prepared at different concentrations. Peritoneal macrophages were obtained from male BALB/c mice and culture cells were stimulated in vitro with propolis or interferon-gamma (IFN-gamma). In the in vivo assay,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propolis; Macrophages; Hydrogen peroxide; Nitric oxide.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302000000200006
Registros recuperados: 42
Primeira ... 123 ... Última
 

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